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Prue Talbot, PhD |
Department of Cell Biology & Neuroscience
2320 Spieth Hall
UC Riverside
Riverside, CA 92521
Phone: (951) 827-3768
Fax: (951) 827-4286
E-Mail: talbot@ucr.edu |
Degrees:
M.A. Biology 1968 Wellesley College
Ph.D. Biology 1972 University of Houston
Research Focus:
My laboratory uses embryonic stem cells to study the effects of environmental toxicants on prenatal stages of development. Each year over 1,000 new chemicals are released into the environment, most without toxicological testing. Embryonic stem cells are potentially valuable models that can be used to identify chemicals that are harmful to prenatal development and thereby provide information that could be used to prevent disease and congenital defects. While much of our prior work has been done using mouse embryonic stem cells, we are currently adapting our assays to human embryonic stem cells which will provide a unique opportunity to directly examine chemicals for toxicity using human cells.
We have recently completed a study comparing the toxicity of mainstream (MS) and sidestream (SS) smoke from traditional and harm reduction cigarettes on attachment of mouse embryonic stem cells to an extracellular substrate and on proliferation of attached cells. Our results showed that filtering MS smoke significantly reduced its toxicity and that SS smoke is far more potent than filtered MS smoke. Unexpectedly, we found that SS smoke from the harm reduction brands, was more potent in the attachment assay that SS smoke from the traditional brand. This finding alerts us to the potential danger of SS smoke from harm reduction cigarettes and shows that MS smoke from harm reduction cigarettes, while perhaps reduced in certain carcinogens, still retains significant toxicity in assays that measure two fundamental processes in embryos, attachment and proliferation.
We are currently following up these studies with additional assays, testing specific chemicals in smoke, and extending our assays to human embryonic stem cells.
Honors/Awards:
Recipient of the Honor's Program Mentor of the Year Award, 2003
Elected Fellow, AAAS, 2001
Recipient of Chancellor's Award for Excellence in Mentoring Undergraduate Research, 1998-99
Recent Teaching:
CBNS 169- Human Embryology
CMDB 200- Cell Biology
CMDB 207- Stem Cell Biology
Discovery Seminar in Stem Cell Biology
Selected Publications:
Pdf files of many of these can be found on the "Publications" link on the left menu.
Lin, S., Vu Tran, P. Talbot
Comparison of toxicity of smoke from traditional and harm reduction cigarettes using embryonic stem cell as a novel model for pre-implantation development.
Human Reproduction 1:1-12 (2009)
Talbot, P.
(2008) In vitro assessment of reproductive toxicity of tobacco smoke and its constituents.
Birth Defects Research (Part C) 84: 61-72.
K. Riveles, V. Tran, Ryan Roza, Derek Kwan, P. Talbot
Smoke from Traditional Commercial, Harm Reduction, and Research Brand Cigarettes Impairs Oviductal Functioning in Hamsters (Merocricetus auratus) In Vitro.
Human Reproduction 2006; doi: 10.1093/humrep/del380
Baibakov, B, L. Gauthier, P. Talbot, Tracy Rankin, and J. Dean (2007)
Sperm binding to the zona pellucida is not sufficient to induce acrosome exocytosis.
Development 134: 933-943.
Richard Yu, M. Wu, S.Lin, Prue Talbot
Cigarette Smoke Toxicants Alter Growth and Survival of Cultured Mammalian Cells.
Toxicological Sciences 2006; 93(1): 82-95.
P. Talbot, L. Fernandez. 2006.
Scrambling the Egg Origin Dogma.
Regenerative Medicine; 1(5): 693-695.
Talbot P, Riveles K.
Smoking and Reproduction: The Oviduct as a Target of Cigarette Smoke.
Reproductive Biology and Endocrinology 2005; 3:25.
Liu M, Oh A, Calarco P, Yamada M, Coonrod SA, and Talbot P.
Peptidylarginine Deiminase (PAD) is a Mouse Cortical Granule Protein that Plays a Role in Preimplantation Embryonic Development.
Reproductive Biology and Endocrinology 2005; 3:24.
Gieseke C, and Talbot P.
Cigarette Smoke Inhibits Hamster Oocyte Pickup by Increasing Adhesion between the Oocyte Cumulus Complex and Oviductal Cilia.
Biology of Reproduction 2005; 73(3): 443-451.
Riveles K, Roza R, Talbot P.
Phenols, Quinolines, Indoles, Benzene, and 2-Cyclopenten-1-ones are Oviductal Toxicants in Cigarette Smoke.
Toxicological Sciences 2005; 86(1): 141-151.
Melkonian, G., Wang, J.L., Chung, J., Munoz, N., Talbot, P.
CD44 and tenascin play critical roles in growth and vascular development of the chick chorioallantoic membrane and are targets of cigarette smoke.
Anat Embryol (2004) 208:109–120.
Riveles, K., Roza R., Arey J., and Talbot, P.. 2004.
Pyrazine derivatives in cigarette smoke inhibit hamster oviductal functioning.
Reproductive Biology and Endocrinology 2:23.
Liu, M., D. Sims, P. Calarco, and P. Talbot. 2003.
Biochemical heterogeneity, migration, and pre-fertilization release of mouse oocyte cortical granules.
Reproductive Biology and Endocrinology 1(1):77. Epub 2003 Nov 07.
Melkonian, G., H. Eckelhoefer, M. Wu, Y. Wang, C. Tong, K. Riveles, and P. Talbot. 2003.
Growth and angiogenesis are inhibited in vivo in developing tissues by pyrazine and its derivatives.
Toxicological Sciences 74: 393-401.
Talbot, P. and P. Dandekar. 2003.
Perivitelline space: does it play a role in blocking polyspermy in mammals?
Microscopy Research and Technique 61: 349-357.
Riveles,K., M. Iv, J. Arey, and P. Talbot. 2003.
Pyridines in cigarette smoke inhibit hamster oviductal functioning in picomolar doses.
Reproductive Toxicology 17: 191-202.
Talbot, P., D. Shur, and D. Myles. 2003.
Cell adhesion and fertilization: Steps in oocyte transport, sperm-zona pellucida interactions, and sperm-egg fusion.
Biology of Reproduction 68: 1-9.
Ji, Lin, G. Melkonian, K. Riveles, and P. Talbot. 2002.
Identification of pyridine compounds in cigarette smoke solution that inhibit growth of the chick chorioallantoic membrane.
Toxicological Sciences 69: 217-225.
Mullick, A., J. McDonald, G. Melkonian, P. Talbot, K. Pinkerton, and J. Rutledge. 2002.
Reactive carbonyls from tobacco smoke increase arterial layer njury.
American Journal of Physiology: Heart, Circulation, and Physiology 283: H591-H597.
Melkonian, G., N. Munoz, J. Chung, C. Tong, R. Marr, and P. Talbot. 2002.
Capillary plexus development in the day 5- day 6 chick chorioallantoic membrane is inhibited by cytochalasin D and suramin.
Journal of Experimental Zoology 292:242-254.
Melkonian, G., L. Chung, R. Marr, C. Tong, and P. Talbot. 2002.
Mainstream and sidestream cigarette smoke inhibit growth and angiogenesis in the day 5 chick chorioallantoic membrane.
Toxicological Sciences 68: 237-248.
Hoodbhoy, T. and P. Talbot. 2001.
Characterization, fate, and function of hamster cortical granule components.
Molecular Reproduction and Development 58: 223-235.
Hoodbhoy, T., P. Dandekar, P. Calarco, and P. Talbot. 2001.
p62/p56 are cortical granule proteins that contribute to formation of the cortical granule envelope and play a role in mammalian preimplantation development.
Molecular Reproduction and Development 59:78-89.
Hoodbhoy, T., E. J. Carroll, and P. Talbot. 2000.
Relationship between p62 and p56, two proteins of the mammalian cortical granules envelope, and hyaline, the major component of the echinoderm hyaline layer, in hamsters.
Biology of Reproduction 62: 979-987
Lam, X., C. Gieseke, M. Knoll, and P. Talbot. 2000.
Assay and importance of adhesive interaction between hamster (Mesocricetus auratus) oocyte cumulus complexes and the oviductal epithelium.
Biology of Reproduction 62: 579-588.
Melkonian, G., C. Lee, W. Zhang, P. Talbot, and M. Martins-Green. 2000.
Normal patterns of angiogenesis and extracellular matrix deposition in chick chorioallantoic membranes are disrupted by mainstream and sidestream cigarette smoke.
Toxicology and Applied Pharmacology, 163: 26-37.
Rankin, T., P. Talbot, E. Lee, and J. Dean. 1999.
Abnormal zonae pellucidae in mice lacking ZP1 results in early embryonic loss.
Development, 126:3847-3855.
DiCarlantonio, G. and P. Talbot. 1999.
Inhalation of mainstream or sidestream cigarette smoke retards preimplantation embryo transport and muscle contraction in vivo.
Biol Reprod., 61(3): 651-656.
Talbot, P., C. Gieseke, and M. Knoll. 1999.
Oocyte pickup by the mammalian oviduct.
Molecular Biology of the Cell 10: 5-8.
Talbot, P., G. Di Carlantonio, M. Knoll, and C. Gomez. 1998.
Identification of cigarette smoke components that alter functioning of hamster (Mesocricetus auratus) oviducts in vitro.
Biology of Reproduction 58: 1047-1053.
Knoll, M. and P. Talbot. 1998.
Cigarette smoke inhibits oocyte cumulus complex pick-up by the oviduct in vitro independent of ciliary beat frequency.
Reprod Toxicol 12: 57-68.
Huang, S., N. Driessen, M. Knoll, and P. Talbot. 1997.
In vitro analysis of oocyte cumulus complex pick-up rate in the hamster Mesocricetus auratus.
Molec Reprod Develop 47: 312-322.
Demers, D., T. Metcalf, P. Talbot, and B. Hyman. 1996.
Multiple lobster tubulin isoforms are encoded by a simple gene family.
Gene 171: 185-191.
Magers, T., P. Talbot, G. DiCarlantonio, M. Knoll, D. Demers, I. Tsai, and T. Hoodbhoy. 1995.
Cigarette smoke inhalation affects the reproductive tract of female hamsters.
Reproductive Toxicology 9: 513-525.
Dandekar, P., K. Mate, and P. Talbot. 1995.
The perivitelline space of marsupial oocytes: extracellular matrix of unfertilized oocytes and formation of a cortical granule envelope following the cortical reaction.
Molec. Reprod. Devel. 41: 363-373.
Knoll, M., R. Shaoulian, T. Magers, and P. Talbot. 1995.
Ciliary beat frequency of hamster oviducts is decreased in vitro by exposure to solutions of mainstream and sidestream cigarette smoke.
Biol. Reprod. 53: 29-37.
DiCarlantonio, G., R. Shaoulian, M. Knoll, T. Magers, and P. Talbot. 1995.
Analysis of ciliary beat frequencies in hamster oviductal explants.
J. Exp. Zool. 272: 142-152.
Talbot, P. and S. Helluy. 1995.
Reproduction and Embryonic Development, In: "Biology of the Lobster", Homarus americanus (ed J. Factor).
Academic Press, pp. 177-216. (Invited book chapter)
Hoodbhoy, T. and P. Talbot. 1994.
Mammalian cortical granules: their contents, fate and function.
Molec. Reprod and Dev., 39: 439-448.
Talbot, P., T. Magers, R. Shaoulian, and M. Knoll. 1994.
Effect of cigarette smoke on axonemal structures.
Molec. Androl. 6: 154-162.
Tsai, I. and P. Talbot. 1994.
Ultrastructural and biochemical analysis of the apical cap: a contractile element of the lobster sperm acrosome.
J. Expt. Zool. 268: 186-199.
Tsai, I. and P. Talbot. 1993.
Video tape analysis of the acrosome reaction of lobster sperm.
Molec. Reprod. Dev. 36:454-461.
Howard, D. and P. Talbot. 1992.
In vitro contraction of lobster (Homarus) ovarian muscle: methods for assaying contraction and effects of biogenic amines.
J. Exp. Zool. 263: 356-366.
Dandekar, P., J Aggelar, and P. Talbot. 1992.
Structure, distribution, and composition of the extracellular matrix of human oocytes and cumulus masses.
Hum. Reprod. 7: 391-398.
Dandekar, P. and P. Talbot. 1992.
Perivitelline space of mammalian oocytes: extracelluar matrix of unfertilized oocytes and formation of a cortical granule envelope following fertilization.
Molec. Reprod. Dev. 31: 135-143.
Talbot, P., H. Al-Hajj, D. Demers, and D. Howard. 1991.
Distribution of microfilaments and microtubules in Homarus pleopod tegumental glands (Crustacea, Decapoda).
Zoomorophology 110: 329-338.
Talbot, P., W. Poolsanguan, B. Poolsanguan, and H. Al-Hajj. 1991a.
In vitro fertilization of lobster oocytes.
J. Exp. Zool. 258: 104-112.
Talbot, P., B. Poolsanguan, H. Al-Hajj, and W. Poolsanguan. 1991b.
Gamete interactions during in vitro fertilization of lobster (Homarus) oocytes.
J. Ultrastructural Res. 106: 125-134.
Talbot, P. and P. Zao. 1991.
Secretion at molting by the pleopod tegumental glands of the lobster Homarus americanus (Milne Edwards).
J. Crust. Biol. 11:1-9.
Talbot, P. and M. Goudeau. 1988.
A complex cortical reaction leads to formation of the fertilization envelope in the lobster, Homarus.
Gam. Res. 19:1-18.
DiCarlantonio, G. and P. Talbot. 1988.
Evidence for sequential deployment of secretory enzymes during the normal acrosome reaction of guinea pig sperm in vitro.
Gam. Res. 21:425-438.
Corselli, J. and P. Talbot. 1987.
In vitro penetration of hamster oocyte-cumulus complexes using physiological numbers of sperm.
Devel. Biol. 122:227-242.
Kooda-Cisco, M. and P. Talbot. 1986.
Ultrastructure and role of the lobster vas deferens in spermatophore formation: the proximal segment.
J. Morph. 188:91-103.
Talbot, P., C. Thaler and P. 1986. Wilson.
Artificial insemination of the American lobster (Homarus).
Gam. Res. 14:25-31.
Ishida, T., P. Talbot and M. Kooda-Cisco. 1986.
Technique for the long term storage of lobster (Homarus) spermatophores.
Gam. Res. 14:183-195.
Schroeder, P. and P. Talbot. 1985.
Ovulation in the animal kingdom: A review with emphasis on the role of contractile elements.
Gam. Res. 11:191-221.
Talbot, P., G. DiCarlantonio, P. Zao, J. Penkala, and L. Haimo. 1985.
Motile cells lacking hyaluronidase can penetrate the hamster oocyte-cumulus complex.
Dev. Biol. 108:387-398.
Harper, R. and P. Talbot. 1984.
Analysis of the epibiotic bacteria of lobster (Homarus) eggs and their influence on the loss of eggs from the pleopods.
Aquaculture 36:9-26.
Talbot, P. and G. DiCarlantonio. 1984.
Ultrastructure of opossum oocyte investing coats and their sensitivity to trypsin and hyaluronidase.
Devel. Biol. 103:159-167.
Kooda-Cisco, M. and P. Talbot. 1983.
A technique for electrically stimulating extrusion of spermatophores from the lobster, Homarus americanus.
Aquaculture 30:221-227.
Kooda-Cisco, M. and P. Talbot. 1982.
A structural analysis of the freshly extruded spermatophore from the lobster Homarus americanus.
J. Morph. 172:193-207.
Talbot, P. and R. S. 1982. Chacon.
In vitro ovulation of hamster oocytes depends on contraction of follicular smooth muscle cells.
Jour. Exp. Zool. 224:409-415.
Schroeder, P. C. and P. Talbot. 1982.
Intrafollicular pressure decreases in hamster preovulatory follicles during smooth muscle cell contraction in vitro.
J. Exp. Zool. 224:417-426.
Talbot, P. 1981.
The ovary of the lobster, Homarus americanus. I. Architecture of the mature ovary.
J Ultrastruct Res. Sep;76(3):235-48.
Talbot, P. 1981.
The ovary of the lobster, Homarus americanus. II. Structure of the mature follicle and origin of the chorion.
J Ultrastruct Res. Sep;76(3):249-62.
Martin, G. and P. Talbot. 1981a.
The role of smooth muscle cells in hamster ovulation.
J. Exp. Zool. 216:469-482.
Martin, G. and P. Talbot. 1981b.
Drugs that block smooth muscle contraction inhibit in vivo ovulation in hamsters.
J. Exp. Zool. 216:483-491.
Talbot P, Chanmanon P. 1980.
The structure of sperm from the lobster, Homarus americanus.
J Ultrastruct Res. Mar;70(3):275-86.
Talbot P, Chanmanon P. 1980.
Morphological features of the acrosome reaction of lobster (Homarus) sperm and the role of the reaction in generating forward sperm movement.
J Ultrastruct Res. Mar;70(3):287-97.
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